| Module-I
Instrumentation Systems- | DNA Sequencing Technologies- PCR, Realtime PCR, PCR-DGGE, Sanger sequencing, Fragment sequencing, Multiplex SNP, typing, Next Gen Sequencing
| 1 week
|
| Module-II
Human Identification (HID) | Laboratory Experiments (hands on): DNA isolation from human samples and quantification, PCR amplification using multiplex primers, fragment (allele) separation following automated capillary electrophoresis, data capturing, data analysis and DNA fingerprint development.
| 2 weeks
|
| Module -III
DNA Barcoding and Genetic Diversity | Laboratory Experiments (hands on): DNA isolation from bio-resource, quantification Specific specific/Breed specific PCR using universal, multiplex primers following by automated DNA sequencing, data capturing, data analysis.
| 3 weeks
|
| Module –IV
DNA based Disease diagnosis | DNA isolations from patients samples (Blood/sputum/serum), DNA quantification, allele specific PCR in disease diagnosis/identification of pathogens, pathogen specific PCR, PCR-RFLP, Identification of viral load, Gene expression studies by Realtime PCR.
| 2 weeks
|
| Module-V
Bioinformatics | Primer Designing, Trace file analysis (Sequence raw file), BLAST, Sequence alignment & annotation, Phylogenetic Tree construction, Genetic Distance calculation, Sequence Sequence Submission to NCBI.
| 1 week |
